發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-12 瀏覽量：408
摘要
? ? ? 對(duì)多種抗菌藥物產(chǎn)生耐藥性的致病菌株的出現(xiàn)是現(xiàn)代醫(yī)療保健中的一個(gè)緊迫問(wèn)題。抗微生物耐藥性主要通過(guò)水平基因轉(zhuǎn)移傳播耐藥性決定因素來(lái)介導(dǎo)。一些耐藥性基因的傳播已經(jīng)有了很好的記錄，但很少有研究分析支撐抗微生物耐藥性基因傳播的模式。分析質(zhì)粒攜帶的抗微生物抗性基因相對(duì)于其宿主基因組的%GC含量%GC含量提供了一種有效檢測(cè)和量化抗微生物耐藥性基因傳播的方法。在這項(xiàng)工作中，我們自動(dòng)化了%GC含量分析，以對(duì)公開(kāi)可用的質(zhì)粒序列中的已知抗微生物耐藥性基因進(jìn)行全面分析。我們發(fā)現(xiàn)抗微生物耐藥性基因的傳播程度主要取決于耐藥性機(jī)制。我們的分析將結(jié)合質(zhì)粒確定為主要傳播載體，并表明最廣泛傳播的基因是從單一基因組背景傳播的。我們發(fā)現(xiàn)，抗微生物藥物的耐藥性傳播情況差異很大，通常反映了管理措施。我們的研究結(jié)果表明，%GC含量分析是一種強(qiáng)大、直觀和可擴(kuò)展的方法，可以使用公開(kāi)的序列數(shù)據(jù)監(jiān)測(cè)耐藥性決定因素的傳播。
Abstract
The emergence of pathogenic strains resistant to multiple antimicrobials is a pressing problem in modern healthcare. Antimicrobial resistance is mediated primarily by dissemination of resistance determinants via horizontal gene transfer. The dissemination of some resistance genes has been well documented, but few studies have analyzed the patterns underpinning the dissemination of antimicrobial resistance genes. Analyzing the %GC content of plasmid-borne antimicrobial resistance genes relative to their host genome %GC content provides a means to efficiently detect and quantify dissemination of antimicrobial resistance genes. In this work we automate %GC content analysis to perform a comprehensive analysis of known antimicrobial resistance genes in publicly available plasmid sequences. We find that the degree to which antimicrobial resistance genes are disseminated depends primarily on the resistance mechanism. Our analysis identifies conjugative plasmids as primary dissemination vectors and indicates that most broadly disseminated genes have spread from single genomic backgrounds. We show that resistance dissemination profiles vary greatly among antimicrobials, oftentimes reflecting stewardship measures. Our findings establish %GC content analysis as a powerful, intuitive and scalable method to monitor the dissemination of resistance determinants using publicly available sequence data.
https://www.mdpi.com/2079-6382/12/2/281