發(fā)布者：抗性基因網(wǎng) 時間：2023-06-12 瀏覽量：390
摘要
目標
鮑曼不動桿菌是一種醫(yī)院內(nèi)病原體，表現(xiàn)出多藥耐藥性，是全世界關(guān)注的主要問題。因此，我們旨在評估臨床菌株鮑曼不動桿菌KBN10P05679的基因組特征，以闡明其抗生素耐藥性機制和毒力因素。
方法
進行了計算機多點序列分型、系統(tǒng)發(fā)育鑒定、基因組注釋、基因組分析、抗生素敏感性測試和生物膜形成分析，并研究了抗生素耐藥性和生物膜相關(guān)基因的表達水平。
后果
KBN10P5679的全基因組包括一個3990428bp的環(huán)形染色體和兩個質(zhì)粒（74294和8731bp），并被分配到ST451序列類型。同源基因簇注釋鑒定了3810個基因，包括參與氨基酸運輸和代謝、轉(zhuǎn)錄、無機離子運輸、能量生產(chǎn)和轉(zhuǎn)化、復(fù)制、重組和修復(fù)以及碳水化合物和蛋白質(zhì)代謝的基因。通過檢索綜合抗生素耐藥性數(shù)據(jù)庫對抗生素耐藥性基因進行了研究，發(fā)現(xiàn)基因組中含有30個不同的抗生素耐藥性基因。對毒力因子數(shù)據(jù)庫的分析揭示了KBN1005679基因組中的86個毒力因子基因。KBN10P5679菌株被發(fā)現(xiàn)具有更高的生物膜形成能力，并且比其他測試菌株以更高的水平表達生物膜相關(guān)基因。
結(jié)論
本研究中獲得的抗生素耐藥性基因型和潛在毒力因子相關(guān)數(shù)據(jù)將有助于指導(dǎo)未來的研究，以制定這種耐多藥病原體的控制措施。
ABSTRACT
Objectives
Acinetobacter baumannii, a nosocomial pathogen, exhibits multidrug resistance and is a major concern worldwide. We therefore aimed to evaluate the genomic features of the clinical strain A. baumannii KBN10P05679 to elucidate its antibiotic resistance mechanisms and virulence factors.
Methods
In silico multilocus sequence typing, phylogenetic identification, genome annotation, genome analysis, antibiotic susceptibility testing, and biofilm formation assay were performed, and the expression levels of antibiotic resistance- and biofilm-related genes were investigated.
Results
The complete genome of KBN10P05679 comprises a circular chromosome of 3 990 428 bp and two plasmids (74 294 and 8731 bp) and was assigned to the ST451 sequence type. Clusters of Orthologous Gene annotation identified 3810 genes, including those involved in amino acid transport and metabolism, transcription, inorganic ion transport, energy production and conversion, replication, recombination and repair, and carbohydrate and protein metabolism. The antibiotic resistance genes were investigated by searching the Comprehensive Antibiotic Resistance Database, and the genome was found to harbour 30 different antibiotic resistance genes. Analysis of the Virulence Factor Database revealed 86 virulence factor genes in the KBN1005679 genome. The KBN10P05679 strain was found to have a higher capacity for biofilm formation and expressed biofilm-related genes at a higher level than the other tested strains.
Conclusions
The antibiotic resistance genotype and potential virulence factor-related data obtained in this study would help direct future studies for developing the control measures for this multidrug-resistant pathogen.
https://www.sciencedirect.com/science/article/pii/S2213716523000322