發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-12 瀏覽量：350
摘要
? ? ? 耐多藥溶血葡萄球菌是醫(yī)院常見的侵襲性菌血癥病原體。我們之前的分析表明，主要菌株之一，源自ST3的ST42，在SH1431的7個基因座中只有一個多點(diǎn)序列分型（MLST）變異；然而在ST42和ST3之間沒有觀察到生物膜形成的顯著差異，這表明其他因素影響克隆譜系的變化。對ST42和ST3的兩個分離株進(jìn)行全基因組測序，以發(fā)現(xiàn)表型和基因型變異，并在140個臨床分離株中進(jìn)一步驗(yàn)證了這些變異。梭形酸和四環(huán)素抗性基因（fusB和tetK）僅在CGMH-SH51（ST42）中發(fā)現(xiàn)。進(jìn)一步的研究顯示，所有分離株的抗性基因型一致，分別有46%和70%的ST42含有fusB和tetK。相反，只有23%和4.2%的ST3分別含有這兩個基因。表型分析還表明，與ST3（分別為23%和4%）相比，ST42分離株對fusidic酸（47%）和四環(huán)素（70%）具有高度抗性。除了耐藥基因外，ST42中的三個膠囊相關(guān)基因的百分比分布高于ST3分離株。我們的研究結(jié)果表明，ST42可能在臺灣流行，需要進(jìn)一步的組成監(jiān)測來防止這種細(xì)菌的傳播。
Abstract
Multi-drug resistant Staphylococcus haemolyticus is a frequent nosocomial invasive bacteremia pathogen in hospitals. Our previous analysis showed one of the predominant strains, ST42 originated from ST3, had only one multilocus sequence typing (MLST) variation among seven loci in SH1431; yet no significant differences in biofilm formation observed between ST42 and ST3, suggesting that other factors influence clonal lineage change. Whole genome sequencing was conducted on two isolates from ST42 and ST3 to find phenotypic and genotypic variations, and these variations were further validated in 140 clinical isolates. The fusidic acid- and tetracycline-resistant genes (fusB and tetK) were found only in CGMH-SH51 (ST42). Further investigation revealed consistent resistant genotypes in all isolates, with 46% and 70% of ST42 containing fusB and tetK, respectively. In contrast, only 23% and 4.2% ST3 contained these two genes, respectively. The phenotypic analysis also showed that ST42 isolates were highly resistant to fusidic acid (47%) and tetracycline (70%), compared with ST3 (23% and 4%, respectively). Along with drug-resistant genes, three capsule-related genes were found in higher percentage distributions in ST42 than in ST3 isolates. Our findings indicate that ST42 could become endemic in Taiwan, further constitutive surveillance is required to prevent the spread of this bacterium.
https://www.mdpi.com/1422-0067/24/7/6198