發(fā)布者：抗性基因網(wǎng) 時間：2023-06-12 瀏覽量：302
摘要
? ? ? 高溫短時巴氏滅菌不足以完全消除牛奶中的芽孢桿菌。因此，評估巴氏滅菌牛奶中它們的流行率、表型和遺傳抗微生物耐藥性是很重要的。在本研究中，我們調(diào)查了芽孢桿菌的患病率及其對25種抗菌藥物的表型耐藥性，以及5種質(zhì)?？咕退幮曰虻幕疾÷?。所有分離株均對β-內(nèi)酰胺類抗生素具有耐藥性；大多數(shù)分離株對氯霉素敏感（n=?52）、環(huán)丙沙星、美羅培南、磺酰胺、四環(huán)素和萬古霉素（n=?56). 有趣的是，盡管表型磺酰胺和四環(huán)素敏感，但在不同的芽孢桿菌物種中檢測到了Sul2和tetA基因，這表明這些可移動抗性基因通過巴氏滅菌牛奶進行水平基因轉(zhuǎn)移的潛在風(fēng)險。
Abstract
High-temperature short-time (HTST) pasteurisation is not sufficient for complete elimination of Bacillus sp. in milk. Hence, it is important to assess their prevalence, phenotypic and genetic antimicrobial resistance profiles in pasteurised milk. In this study, we investigated Bacillus prevalence and their phenotypic resistance to 25 antimicrobials and prevalence of five plasmid antimicrobial resistance genes. All isolates were resistant to β-lactams; most isolates were susceptible to chloramphenicol (n =?52), ciprofloxacin, meropenem, sulphonamides, tetracycline and vancomycin (n =?56). Intriguingly, despite phenotypic sulphonamide and tetracycline sensitivity, Sul2 and tetA genes were detected in different Bacillus species, signifying a potential risk of horizontal gene transfer of these mobile resistance genes through pasteurised milk.
https://onlinelibrary.wiley.com/doi/abs/10.1111/1471-0307.12919