發(fā)布者：抗性基因網(wǎng) 時間：2023-06-12 瀏覽量：427
摘要
? ? ? 類似飲食的攝入導(dǎo)致了不同系統(tǒng)發(fā)育動物腸道微生物組成和功能的趨同。然而，考慮到腸道微生物組的功能冗余，目前尚不清楚它們的功能是否只同步，而不是在它們的組成中同步，即使是在食用類似飲食的系統(tǒng)發(fā)育接近的動物中也是如此。在這項(xiàng)研究中，我們在2021年4月（食物供應(yīng)前）和2021年6月和12月（食物提供后）從Rhinoopithecus roxellana種群中收集了新鮮糞便樣本，并使用高通量測序方法（全長16S rRNA基因測序和宏基因組）來研究食物供應(yīng)引起的腸道微生物組的變化。結(jié)合我們之前對野生鼻古猿種群的研究結(jié)果，我們發(fā)現(xiàn)人工食物（如蘋果、胡蘿卜和花生）會影響腸道微生物組，并且在這兩個鼻古猿物種中，只有其功能和抗生素抗性基因群落發(fā)生同步，反映了其生態(tài)功能的冗余。鑒于目前的發(fā)現(xiàn)（例如，益生菌的耗竭、腸道微生物群落的微生態(tài)失調(diào)以及抗生素耐藥性基因譜的變化），人為干擾（例如，食物供應(yīng)）將對宿主健康產(chǎn)生潛在的負(fù)面影響。因此，應(yīng)該從腸道微生物多樣性的角度重新思考人類在動物保護(hù)方面的活動。
Abstract
The consumption of similar diets has led to the convergence of gut microbial compositions and functions across phylogenetically distinct animals. However, given the functional redundancy in gut microbiomes, it remains unclear whether synchrony occurs in their functions only and not in their composition, even within phylogenetically close animals consuming a similar diet. In this study, we collected fresh fecal samples from a Rhinopithecus roxellana population in April 2021 (before food provisioning) and June and December 2021 (after food provisioning) and used high-throughput sequencing methods (full-length 16S rRNA gene sequencing and metagenomes) to investigate changes in the gut microbiome due to food provisioning. Combining the results from our previous studies on a wild Rhinopithecus bieti population, we found that the artificial food provisions (e.g., apples, carrots, and peanuts) affected the gut microbiome, and synchrony occurred only in its functions and antibiotic resistance gene community in both Rhinopithecus species, reflecting its ecological functional redundancy. Given the current findings (e.g., depletion in probiotic microbes, dysbiosis in the gut microbial community, and changes in the antibiotic resistance gene profile), anthropogenic disturbances (e.g., food provisioning) would have potential negative effects on host health. Therefore, human activity in animal conservation should be rethought from the standpoint of gut microbial diversity.
https://www.sciencedirect.com/science/article/pii/S0048969722070577