發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-12 瀏覽量：558
摘要
? ? ? 南極微生物是進(jìn)化適應(yīng)的重要因素，也是生物活性化合物的自然資源，具有生物合成天然產(chǎn)物的特殊代謝途徑。然而，對(duì)南極地區(qū)魚(yú)類的共生微生物群知之甚少。在本研究中，進(jìn)行了培養(yǎng)方法和全基因組測(cè)序。進(jìn)行了天然產(chǎn)物分析，以確定生物合成潛力。我們報(bào)道了一種共生細(xì)菌Serratia myotis L7-1的分離和鑒定，該細(xì)菌具有高度適應(yīng)性，并存在于南極魚(yú)類伯氏銀耳中?；蚪M分析顯示，南極菌株S.myotis L7-1具有碳水化合物活性酶（CAZymes）、生物合成基因簇（BGCs）、應(yīng)激反應(yīng)基因、抗生素抗性基因（ARGs）和完整的IV型分泌系統(tǒng)，可以促進(jìn)在極端南極環(huán)境中的競(jìng)爭(zhēng)和定殖。微生物組基因簇的鑒定表明了生物活性化合物的生物合成潛力?；谏锘钚砸龑?dǎo)的分級(jí)純化，serranticin被鑒定為生物活性化合物，顯示出顯著的抗菌和抗腫瘤活性。serranticin基因簇被鑒定并定位在鉻上。此外，多藥耐藥性和強(qiáng)大的細(xì)菌拮抗作用有助于在生態(tài)位中發(fā)揮競(jìng)爭(zhēng)優(yōu)勢(shì)。我們的研究結(jié)果強(qiáng)調(diào)了南極魚(yú)類中共生細(xì)菌的存在，這種共生細(xì)菌主要以生物活性天然產(chǎn)物為代表，并具有在南極海洋魚(yú)類中生存的適應(yīng)性。
Abstract
Antarctic microbes are important agents for evolutionary adaptation and natural resource of bioactive compounds, harboring the particular metabolic pathways to biosynthesize natural products. However, not much is known on symbiotic microbiomes of fish in the Antarctic zone. In the present study, the culture method and whole-genome sequencing were performed. Natural product analyses were carried out to determine the biosynthetic potential. We report the isolation and identification of a symbiotic bacterium Serratia myotis L7-1, that is highly adaptive and resides within Antarctic fish, Trematomus bernacchii . As revealed by genomic analyses, Antarctic strain S. myotis L7-1 possesses carbohydrate-active enzymes (CAZymes), biosynthetic gene clusters (BGCs), stress response genes, antibiotic resistant genes (ARGs), and a complete type IV secretion system which could facilitate competition and colonization in the extreme Antarctic environment. The identification of microbiome gene clusters indicates the biosynthetic potential of bioactive compounds. Based on bioactivity-guided fractionation, serranticin was purified and identified as the bioactive compound, showing significant antibacterial and antitumor activity. The serranticin gene cluster was identified and located on the chrome. Furthermore, the multidrug resistance and strong bacterial antagonism contribute competitive advantages in ecological niches. Our results highlight the existence of a symbiotic bacterium in Antarctic fish largely represented by bioactive natural products and the adaptability to survive in the fish living in Antarctic oceans.
https://www.osti.gov/biblio/1909301