發(fā)布者：抗性基因網(wǎng) 時間：2023-06-12 瀏覽量：379
摘要
介紹
眾所周知，植物微生物群可以保護(hù)宿主免受植物病原體的入侵。最近的研究表明，室內(nèi)植物的微生物群會傳播到當(dāng)?shù)氐慕ㄖh(huán)境中，在那里它可能會實現(xiàn)尚未探索的功能。更好地了解這些微生物群落與人類病原體的相互作用，可能會提供與它們的自然抑制有關(guān)的新線索。
客觀的
我們研究了兩種室內(nèi)模式植物——穆薩和吊蘭的植物微生物群，以及它們對人類病原體的影響。主要目的是確定室內(nèi)植物微生物群抑制人類致病菌的機(jī)制。
方法
使用一套綜合實驗和方法，結(jié)合擴(kuò)增子和鳥槍宏基因組分析以及相互作用分析的結(jié)果，對微生物群落和功能進(jìn)行了研究。
后果
在不同的室內(nèi)環(huán)境中生長的穆薩和吊蘭上發(fā)現(xiàn)了不同的微生物群落；數(shù)據(jù)集包括1066個細(xì)菌ASV、1261個真菌ASV和358個古生ASV。細(xì)菌群落對每種植物都是特定的，而真菌和古生菌群落主要是由建筑環(huán)境形成的。鞘氨醇單胞菌和芽孢桿菌被發(fā)現(xiàn)是這兩種模式植物中普遍存在的核心微生物組的主要成分；眾所周知，它們對植物病原體具有拮抗活性。相互作用分析表明，它們也可以拮抗人類機(jī)會性病原體。此外，本地植物微生物群含有廣泛的生物合成基因簇，同時還含有多種抗微生物耐藥性基因。通過對植物和非生物表面進(jìn)行比較宏基因組分析，我們發(fā)現(xiàn)葉層微生物群具有與周圍非生物表面明顯不同的特征。
結(jié)論
自然存在的葉層細(xì)菌有可能成為抵御人類機(jī)會性病原體的保護(hù)屏障。這些知識和潛在的機(jī)制可以為在建筑環(huán)境中建立健康的微生物組提供重要的基礎(chǔ)。
圖形摘要
室內(nèi)植物相關(guān)細(xì)菌作為抵御人類機(jī)會性病原體的防線的發(fā)現(xiàn)意義的示意圖可視化。省略號文本：本研究證實了細(xì)菌群落的所有相關(guān)功能，這些功能有助于屏蔽病原體。矩形文本：用于分析各自功能的方法和技術(shù)。
Abstract
Introduction
The plant microbiota is known to protect its host against invasion by plant pathogens. Recent studies have indicated that the microbiota of indoor plants is transmitted to the local built environment where it might fulfill yet unexplored functions. A better understanding of the interplay of such microbial communities with human pathogens might provide novel cues related to natural inhibition of them.
Objective
We studied the plant microbiota of two model indoor plants, Musa acuminata and Chlorophytum comosum, and their effect on human pathogens. The main objective was to identify mechanisms by which the microbiota of indoor plants inhibits human-pathogenic bacteria.
Methods
Microbial communities and functioning were investigated using a comprehensive set of experiments and methods combining amplicon and shotgun metagenomic analyses with results from interaction assays.
Results
A diverse microbial community was found to be present on Musa and Chlorophytum grown in different indoor environments; the datasets comprised 1066 bacterial, 1261 fungal, and 358 archaeal ASVs. Bacterial communities were specific for each plant species, whereas fungal and archaeal communities were primarily shaped by the built environment. Sphingomonas and Bacillus were found to be prevalent components of a ubiquitous core microbiome in the two model plants; they are well-known for antagonistic activity towards plant pathogens. Interaction assays indicated that they can also antagonize opportunistic human pathogens. Moreover, the native plant microbiomes harbored a broad spectrum of biosynthetic gene clusters, and in parallel, a variety of antimicrobial resistance genes. By conducting comparative metagenomic analyses between plants and abiotic surfaces, we found that the phyllosphere microbiota harbors features that are clearly distinguishable from the surrounding abiotic surfaces.
Conclusions
Naturally occurring phyllosphere bacteria can potentially act as a protective shield against opportunistic human pathogens. This knowledge and the underlying mechanisms can provide an important basis to establish a healthy microbiome in built environments.
Graphical abstract
Schematic visualization of the discovered implications of indoor-plant-associated bacteria as a defense line against opportunistic human pathogens. Text in ellipses: All relevant functions of the bacterial community confirmed in this study that serve to shield off pathogens. Text in rectangles: Methods and techniques that were implemented to analyze the respective functions.
https://www.sciencedirect.com/science/article/pii/S2090123222000467